UQ Resource Centre
Quality-assured plasmids, expression strains, cell lines, media and in-house enzymes, supplied to the UQ research community to accelerate molecular cloning, recombinant expression, purification and protein modification workflows.
Catalogue
All materials are produced and quality-controlled in-house under the NBF ISO 9001 quality management system. Available as streaked plates, glycerol stocks, minipreps, frozen vials or working stocks depending on item.
Plasmid Library
A curated collection of expression vectors covering every supported host system, with affinity, solubility, fluorescent and protease-cleavable tag options.
- pOPIN series: NHis, NHis3C, TRX, SUMO, GST, NusA
- Fluorescent variants: EGFP, ECFP, EBFP, Citrine, mCherry
- Bacterial: pET, pGEX, pDEST series
- Insect (BEVS): pFastBac variants, pBac-1
- Mammalian: pcDNA3.1
Bacterial Strains
Cloning and expression strains, supplied as streaked plates or chemically competent cells ready for transformation.
- Cloning hosts: XL1-Blue, Top10, JM109, DH5α
- Expression hosts: BL21(DE3) and variants (pLysS, CodonPlus-RIL, Star)
- Specialty strains: Rosetta, Arctic Express (DE3) pLysS
- Disulfide-friendly: Shuffle T7 Express, Tuner (DE3)
Insect & Mammalian Cells
Authenticated, mycoplasma-tested cell lines for baculovirus and mammalian expression workflows.
- Insect: Sf9 (Spodoptera frugiperda)
- Insect glycoengineered: Mimic Sf9 (mammalian glycosyltransferases)
- Insect alt: High Five™ (Trichoplusia ni)
- Mammalian: CHO and HEK293 cell lines
Yeast & Media
Methylotrophic yeast strains for high-density secreted protein production, plus speciality insect cell media.
- Pichia strains: PichiaPink (Strains 1–4)
- Pichia alt: GS115, X-33, KM71H
- Insect cell media: ESF 921
In-House Produced Enzymes
NBF manufactures a panel of molecular biology and protein-modification enzymes in-house, supplied at a fraction of commercial prices to the UQ research community. Each batch is QC-tested for activity, purity and contaminating nucleases or proteases.
Accessing the Resource Centre
UQ staff and students can request quality-assured materials directly from NBF. Pricing, ordering and delivery details are provided on enquiry. External academic and industry users should contact NBF to discuss bespoke supply arrangements.
Contact NBF
